FastQCFastQC Report
Mon 19 Sep 2022
EGAF00000056278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000056278
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences38050132
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT7237711.9021510884640294No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG3571790.9387063361567314No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA2431030.6389018571604429No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTG2350160.6176483172252859No Hit
CGCGACCTCAGATCAGACGTGGCGCCTTGGCCGTA2133850.5607996313915546No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1872910.49222168270007577No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA1737910.4567421737196602No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1457920.38315767209427815No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1374490.3612313355443813No Hit
GCATTGGTGGTTCAGTGGTAGAACCTCTATTCCAA1254530.3297045066755616No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCTTGG1199470.3152341232351047No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA1132160.2975443028686471No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG997550.2621672902475082No Hit
GCTAAACCTAGCCCCAAACCCACTCCGCCTTGGCC994060.26125007923757004No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN938340.24660624041987553No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA872410.22927909947855107No Hit
TAGCTTATCAGACTGAGGTTGACGCCTTGGCCGTA856720.22515559210149388No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCC778690.20464843591081366No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN744340.1956208719591301No Hit
GTTTCCGTAGTGTAGTGGTCATCACATTCGCCTTG666800.1752424932454899No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA628350.16513740346551228No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT609600.160209693884899No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTACC599060.15743966407265028No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG597070.15691666982916117No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTA593150.15588645001284096No Hit
CGCCTTGGCCGTACAGCAGAAGGGGAGAGAGAAGA586170.15405202799296466No Hit
TAGCTTATCAGACTGATGTCGACCGCCTTGGCCGT585100.15377082003289766No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCTTGG567710.14920053365386485No Hit
GCATTGGTGGTTCAGTGGTAGAACGAGATAAGGTT548890.14425442729081728No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTG509120.13380242675636447No Hit
TAGCTTATCAGACTGACGTTGACGCCTTGGCCGTA505560.13286681896399205No Hit
AGCTTATCAGACTGATGTTGACCGCCTTGGCCGTA494460.12994961489226897No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG478130.12565790836152685No Hit
GCATTGGTGGTTCAGTGGTAGAATCCTCGCCTTGG466360.12256462080078986No Hit
TAGCTTATCAGACTGATGTTAACCGCCTTGGCCGT462270.1214897230842721No Hit
GCATTGGTGGTTCAGTGGTAGGATTCTCGCCTTGG433430.11391024872134478No Hit
TAGCTTATCAGACTGAAGTTGACGCCTTGGCCGTA416280.10940303702494383No Hit
CAGTGCAATGTTAAAAGGGCATCGCCTTGGCCGTA408700.1074109282984879No Hit
CGCGACCTCAGATCAGACGTGGCCGCCTTGGCCGT390100.10252264039451951No Hit
GCATTGGTGGTTCAGTGGTAGAATAGAGCGGAACC384740.10111397248240822No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACCTGCC383870.10088532675786777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)