FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000056287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000056287
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences10001034
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTTGGCCGTACAGCAGCCGATGAGAGAATGAG400350.40030860808992347No Hit
CGCGACCTCAGATCAGACGTGGCGCCTTGGCCGTA355830.35579321098198446No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT320550.3205168585568252No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT266210.2661824767319059No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA235280.23525567456325014No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTTTAATGC203120.20309899956344515No Hit
ACCCCATAAACACCACGCCTTGGCCGTACAGCAGC187790.18777058452156048No Hit
CGCCTTGGCCGTACAGCAGCCGATGAGAGAATTCT171830.1718122346149408No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTTTAATTA145240.14522498373668163No Hit
TGGCTCAGTTCAGCAGGGACAGTCGCCTTGGCCGT132370.1323563143570955No Hit
ATGTCAAAGTGAAGAAATTCAATGAAGCGCCTTGG129800.129786580067621No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA127490.12747681889692605No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTTTAAAAT123790.12377720143737139No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTA115200.11518808955154036No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)