FastQCFastQC Report
Mon 19 Sep 2022
EGAF00000056339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000056339
File typeColorspace converted to bases
EncodingSanger / Illumina 1.9
Total Sequences34760346
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG4569491.3145697686668596No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG1828050.5259009792365128No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCCTTGG1663160.478464742554634No Hit
CGCGACCTCAGATCAGACGTGGCGCCTTGGCCGTA1525890.4389743416247928No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT1480050.42578690097043337No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1472590.42364077733863753No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCGCC1422620.4092652012152008No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTG1282160.36885708790125393No Hit
GCATTGGTGGTTCAGTGGTAGGATTCTCGCCTTGG1149270.3306267434737272No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTA1109370.3191481465690819No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1010990.29084578156960805No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT925920.26637249237967886No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN917810.2640393740614665No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCC870550.2504434219383202No Hit
CGCGACCTCAGATCAGACGTGGCGACGCCTTGGCC782410.22508694246023905No Hit
ATGTCAAAGTGAAGAAATTCAATGAAGCGCCTTGG771550.22196269277641828No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTG757370.21788333177120847No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA737280.21210375754027302No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTC709200.20402558708707905No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN696300.20031446177204335No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCTTG621790.17887911702605033No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGCCT581140.16718475702169364No Hit
GCATTGGTGGTTCAGTGGTAGAACCTCTATTCCAA581100.1671732496563757No Hit
CGCGACCTCAGATCAGACGTGGCCGCCTTGGCCGT523680.1506544267424726No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTC518930.1492879271109672No Hit
CGCCTTGGCCGTACAGCAGCCGATGAGAGAGACTC507530.14600832799535424No Hit
GCATTGGTGGTTCAGTGGTAGAATCCTCGCCTTGG506150.14561132389188533No Hit
TACCTGGTTGATCCTGCCCTGCTTGCGCATCCACT492000.14154059341066397No Hit
TACCTGGTTGATCCTGCCCTGCTACGCGTAGGTGA476690.1371361493352224No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA473360.13617816117250386No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCCTTGG437470.12585317764098206No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTTG430460.12383651186901304No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTTG424910.12223986493114884No Hit
GTTTCCGTAGTGTAGTGGTCATCACATTCGCCTTG417650.1201512781259427No Hit
TAGCTTATCAGACTGAGGTTGACGCCTTGGCCGTA411830.11847695647218243No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCC397190.11426526076581632No Hit
GCATTGGTGGTTCAGTGGTAGTTAAGAGCGGAACC395230.11370139986523725No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCGCCTT391470.11261970752535087No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA383440.11030960393777439No Hit
AAACCGTTACCATTACTGAGTTTCGCCTTGGCCGT369320.1062475039805415No Hit
CGCGACCTCAGATCAGACGTGGCAACCCGCCTTGG361740.10406685825279186No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG361460.10398630669556626No Hit
GCATTGGTGGTTCAGTGGTAGAACTCTCGCCTTGG355210.10218828086463812No Hit
CGCGACCTCAGATCAGACGTGGCGACCGCCTTGGC350920.10095411593428903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)