FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068531
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences13315631
Sequences flagged as poor quality0
Sequence length40
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................342560.2572615597413296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAA...350.001453053724.2857132
.TTTCCC450.006242311618.888891
GCGGTTC10600.016.3584931
GTT....1001.3133789E-515.3000011
ATGCCGA11350.015.27753314
AATGCCG12550.014.35856513
GATCGGA11900.014.28571421
AGCGGTT12250.014.01632730
GAGCGGT12700.013.78740129
ATCGGAA12900.013.31007822
AGATCGG13750.012.610908520
CAA....1503.3795695E-612.4666661
TGCCGAG14350.012.43902515
CAG....2201.0895747E-912.3636361
AAGAGCG14250.011.81052727
TCGGAAG14350.011.72822323
AGAGCGG15100.011.59602628
CTA....1352.1922527E-411.3333341
CGGTTCA16650.011.2312311
TAA....2151.2999953E-711.0697683