FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068533
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences14735279
Sequences flagged as poor quality0
Sequence length40
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................645960.4383764976557281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAT..200.002790051534.04
.C...G.305.908995E-428.33333227
C...T..250.00830905827.226
...TTTC250.00830905827.21
....GGG350.001453060924.2857131
GGGA...504.7871086E-420.4000022
...TG..504.7871086E-420.40000222
....TG.504.7871086E-420.40000221
....ATT450.006242342818.8888874
GGCA...600.00162964217.02
..TG...600.00162964217.023
ATGCCGA10400.016.34615314
GCGGTTC10650.015.16431931
AATGCCG11900.014.71428613
AGCGGTT11500.014.33913130
GAGCGGT12350.013.35222729
GATCGGA12650.013.03557221
AGAA...1053.39277E-412.9523812
.....AT1605.0014205E-712.753
TGCCGAG13800.012.68840615
Produced by FastQC (version 0.11.5)