FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068559
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences18734482
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232000.12383582316287155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCA3601.7527613E-49.93790268
ACGACGC4501.6865222E-48.74535368
TGCGCGA4100.00620643477.859909569
TCGCGCG4600.00197093387.79510870
GACGACG4200.0078364947.65787467
CGCGACG4200.0086218017.576470464
CGCGGAC6351.0998051E-47.330414369
AACGCGG6451.3475746E-47.210756368
CGCAGCG9255.8647584E-66.58060969
TCGGCGC7004.2017252E-46.571609565
CGCGGCG14656.2755134E-96.119026770
GTGCGCG10052.1431812E-56.05173768
ACGCGCG7150.00377569235.94336166
GCGGACG7300.00414020575.89438370
TGCGCGG9554.1401005E-45.632067770
CGCGCGG15551.5069418E-75.515639367
ACGGGCG9655.6389306E-45.50453866
AGCGCGC10008.0503203E-45.360511367
CGCGCAC12207.0038834E-55.282890369
CACGCCG10250.00107046045.247439470
Produced by FastQC (version 0.11.5)