FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068560
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences18145540
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN228500.12592626066791068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG3950.00455941268.13433370
GCGGACG6854.4645276E-67.80560469
CGCGAGA5551.969556E-47.707154369
CGCGACG5106.754674E-47.688264469
CGTGCGG8702.9982948E-77.386347370
CGCGCGA5350.0092646436.64962467
CGGCGAA6400.00118206786.590456553
GGGTCGA9052.1621666E-45.908108769
GCGTGCG9951.2655932E-45.73195469
GCGCGAG11302.2556103E-55.67298368
ACGCGGG14853.552923E-75.529398470
CGCTGCG8550.00415435155.428173570
GCGCGCG16559.8983946E-85.392851470
CAGGTCG8650.0047640825.3654270
GCATCGA9400.00217893675.31712270
CGCGCGC15353.875104E-65.116709770
GACGCGG10650.00197622885.01063267
Produced by FastQC (version 0.11.5)