FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068569
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences17093078
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................732710.4286588992339472No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC634090.3709630295959569Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAG632030.3697578633877409Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
...ACTG200.002790063234.0000042
TTTCT..305.9090316E-428.3333361
CAGGA..305.9090316E-428.3333361
GCGGTTC166100.015.81276411
AGCGGTT168500.015.5471810
GAGCGGT169400.015.4946869
ATGCCGA163750.015.25068714
AAGAGCG175800.015.2207067
AATGCCG165700.015.20458713
AGAGCGG177400.015.1409258
TGCCGAG166800.014.99220615
AGATCGG164350.014.97779220
CGGAAGA185600.014.6002164
ACCGATA5850.014.23931533
CGAGATC175150.014.13188718
CCGAGAT179000.013.80893817
GAGATCG181750.013.64676819
GACCGAT7350.013.64625932
GCCGAGA186900.013.50722316
GAATGCC188250.013.47357312
Produced by FastQC (version 0.11.5)