FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068570
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences17093078
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................2774691.6232828282887377No Hit
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTA573020.3352351168116123Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
....ACC200.002790063234.00000411
...TCTG200.002790063234.00000433
CGGCATT99350.031.5873181
ACCGAGA75750.030.63366316
GAACCGA79750.030.56802414
CGCGGGT18350.030.29427718
AACCGAG80600.030.26674815
TGAACCG102450.030.23328213
AACCGCG18600.029.88709615
ACCGCGG19750.029.61012816
GCGTCGT73650.029.52206231
CGTCGTG74100.029.29689432
TCGTGTA72950.028.98971734
AGCGTCG76900.028.7386230
GTCGTGT75000.028.46933233
GCGGGTA21000.028.33333419
GTACGAG13100.027.90076423
GAGCGTC80550.027.4574829
TGC....250.00830909427.21
GGTACGA13950.027.17562922
Produced by FastQC (version 0.11.5)