FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068571
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences17382005
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................672010.3866124765238533No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC639940.36816236101646505Illumina Paired End PCR Primer 2 (96% over 25bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAG630340.3626394078243563Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
..GGCAA200.002790065234.01
..ACTTT200.002790065234.01
...GCTC200.002790065234.02
..CTGTG200.002790065234.01
.ATCGGA305.909037E-428.3333321
..TTTTT250.00830909927.21
TAAAAA.250.00830909927.21
.GA....400.003157433421.2523
GACCGAT5550.018.37837832
ACCGATA5700.017.033
GCGGTTC158700.015.70384511
AGCGGTT161200.015.56575710
GAGCGGT161800.015.5500619
AGAGCGG168550.015.1492148
ATGCCGA157300.015.08709514
AAGAGCG169600.015.0654487
AATGCCG159600.014.901628513
AGATCGG157300.014.8601420
TGCCGAG160250.014.81996815
CGGAAGA178700.014.3743714
Produced by FastQC (version 0.11.5)