FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068583
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19783344
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN242860.12275983271584419No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG2050.001722948513.05821770
ACGCGAC3650.0015400299.16958669
CGCGCGG9358.62201E-108.34764268
ACGCGCG5802.2196637E-58.33518969
GCGCGTC6902.742805E-68.07738267
GCGGTCG4200.0055130747.96433168
GCGACGC5600.00117226917.300637768
CGCTCGC6305.2003784E-47.08338469
CGCGACA5450.00550844157.016865770
CTGCGCG9805.173195E-66.63380270
GCTCGCG7705.2090525E-46.456428570
ACGCGGA6950.00159711916.417303668
CGCGCGC11601.6456343E-66.26376370
CGTCGTG7757.902357E-46.237948469
ACGGCGC8109.609263E-46.137592370
GGCGACG6900.00776705566.09653870
CGGCGCG10202.0010672E-56.07881165
GCGTCGT7450.0042961635.874824565
CACGCGA8250.00168598655.856668
CACGCGG14053.305595E-75.81801467
Produced by FastQC (version 0.11.5)