FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068584
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19382501
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN281930.1454559450300041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG2700.001383889810.95609165
TCTCGCG6708.024097E-46.81920769
GACGCGG10151.11105055E-56.31520670
GCGCGGA7550.00344161785.992926670
CGGTGCG7950.0049227455.803284668
CCGCGCA9702.9633028E-45.77280766
CGCGCGG10551.3095158E-45.718369570
TGAACCG11501.6623666E-55.463979713
GCGCGCG15251.2093533E-65.43948270
CGCGCAG9300.0052969135.317239867
GCGCACG11454.212794E-45.26889170
CCGCGCG10350.00366808575.100260770
Produced by FastQC (version 0.11.5)