FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068621
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19750054
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG16000.013.50497613
AGCGTCG15350.010.66369830
GAACCGA17650.010.66168514
CGGCATT21400.010.60451
GCGTCGT13550.010.2811431
CGTCGTG14500.09.84761232
ACCGAGA18850.09.79743916
AACCGAG20200.08.79803515
TAGATCG13950.08.56261454
TCGTGTA18950.07.534192634
GAGCGTC23200.07.0559229
GTCGTGT20450.06.982165333
GCGGACG5700.00227445536.857246470
ACCGCGG7852.3219356E-46.337096770
CGGCGTG10508.363408E-66.033550367
TCGGTGG21000.05.909186458
GCGGCGT9052.5768782E-45.8324866
CGTGTAG24550.05.815613735
CCGCGCG9050.00161695145.44464167
GATCGGA29250.05.358232521
Produced by FastQC (version 0.11.5)