FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068623
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19649902
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN328760.16730872245571504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG7107.559107E-56.90695867
CAGCGCG10201.1046723E-76.874428370
AGCGTCG7702.9832501E-56.820430865
ATGCGCG5150.00749836026.796983764
CTGCGCG8802.7057567E-56.374469870
CGGCGCG8257.624428E-56.374469370
GCGTCGT6950.00311397026.04686366
AGCGGCG12303.209594E-65.69902169
CGCGGCG9200.00208824385.335154570
GCGCGAG9500.00313337245.16511369
CCGCGCG9050.0099903175.036183470
Produced by FastQC (version 0.11.5)