FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068663
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19625925
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN285240.14533837258626026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG8101.4551915E-119.56010865
GCGTCGT7151.3897079E-99.35629766
CGTCGTG7756.166374E-109.08803567
TAGATCG9704.3475666E-87.23361854
GCGCGGC6854.3852946E-57.19887269
GTCGTGT12752.120396E-86.353158568
GAGCGTC13405.9641025E-86.04000364
Produced by FastQC (version 0.11.5)