FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068671
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19312170
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230770.11949459848375403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG3551.0331878E-612.00825370
CGCGTCG3200.0078640328.88110470
CGTCGGG5251.1263823E-58.79652270
ACGCCGA4157.9098815E-48.54926569
CGACACG4350.00126115128.15619669
GCGCGTC5552.1143492E-47.66001867
GCGCGAC6156.871268E-46.912698367
ACGCGCG7550.00100104736.11678770
CGCGCTG8506.4812665E-45.851080470
CTCCGCG9152.7871408E-45.79892565
CTGCGCG8607.5250055E-45.78304570
CGGAGCG9705.7938584E-45.49346770
CGCCGCG11151.2071112E-45.40151567
CGCGCGC13902.1530503E-55.11142770
CGGCGTG9750.00366208215.100941770
Produced by FastQC (version 0.11.5)