FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068672
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19541515
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN250780.12833191285322557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG3850.00386822958.28408468
GCGGCGT9603.596142E-97.758490669
AGCGTCG6853.2620173E-46.709521365
GCGTCGT6500.00130803526.531761666
CCGCGCG13306.3064363E-96.409057670
AGCGCGG11407.01104E-76.20708366
GACGCGC6300.00671559676.18271667
GCGCGTC6450.0084267766.04870269
CGCGTGT8305.0819304E-45.96332865
GCGAGCG11404.3150976E-65.919476570
ACGGCGC8000.0021895445.729682461
GCGCGGA8100.00239077025.687495768
CGCGGCG15753.9008228E-85.629743669
CGGGACG7750.0089194795.49938570
TCGCGGG8500.00412011855.4319970
GCCGCGC13803.4240566E-65.39721169
GCGCGCC15852.549241E-75.377947370
GCGCGCG18751.0297299E-85.30385170
CGCGCGC16254.26915E-75.245567370
CGCGCTG9550.00294355755.1910167
Produced by FastQC (version 0.11.5)