FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068674
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences16728336
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN234950.1404503113758595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG3102.8608592E-612.65363865
TCGCGCA3300.0093811098.69179869
CGGCGTC5250.00705706246.83973870
CGCGTGC6600.00137765296.501857367
GACGCGC8256.028459E-56.48368265
GGACGCG12851.3239696E-76.09772164
ACGCGGC8200.00244180355.67738468
CGCGCGG20658.54925E-115.56453370
GTCTGCG11008.54707E-55.526578467
GCGCGCG23101.0913936E-115.432374569
CGCGCAG10108.624927E-45.33296470
GCGAGCG9550.0024708965.264091570
CGCGCTG8850.0060087125.26040168
TCTGCGC12358.4741034E-55.219444868
ACGCTCA9650.00329586515.1442260
GCGCGAG9850.00363681165.10376470
GGCGCGC31150.05.058402568
CGCCGCG17854.8182665E-75.008433367
Produced by FastQC (version 0.11.5)