FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068698
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences28501477
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................3833211.3449162652167115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
..TACTC200.00279009534.012
.GT....501.6743797E-830.59999822
.TCA...354.2577914E-529.14285721
...AA.A305.9091294E-428.33333634
.TTC...305.9091294E-428.33333621
.CCC...305.9091294E-428.33333621
..ATTTT305.9091294E-428.33333612
..GT...554.2284228E-827.8181821
ATAT...250.00830918927.19999922
T....AG250.00830918927.19999917
.AAA...250.00830918927.19999921
..TTC..250.00830918927.19999920
.A..A..250.00830918927.19999928
..CCC..250.00830918927.19999920
TT....A250.00830918927.19999916
.AGA...250.00830918927.19999921
..TG...1100.026.27272621
..GA...657.385097E-926.15384521
...GT..609.8285454E-825.50000220
..TT...1500.024.93333221
Produced by FastQC (version 0.11.5)