FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33842635
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG6750.011.97078311
GAGGTAA110800.09.0149451
GTCGCGC8800.08.9339712
GGGGTAA55700.08.5936091
AGGTAAT120750.08.5010882
TATTGCG14950.08.245831527
GTCGACT19850.08.10240532
CAGGTAA188800.07.571161
AGTCGAC22200.07.5423931
GAGGTAG93950.07.46783831
GAGAACC113200.07.39501431
CCGTCGC11600.07.342285610
AGGTAAA181300.07.3243582
AGGTAAC88050.07.26778462
CAAGTAA109150.07.10871841
CAGGTAG121000.07.08088251
TCGCGCC11600.07.075055613
GATTACC53950.07.00876241
GAAGTAA127200.06.97630741
GCTTAAT84850.06.7433359
Produced by FastQC (version 0.11.5)