FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068705
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences16879217
Sequences flagged as poor quality0
Sequence length40
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................2535541.5021668362934135No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1566030.9277859275107371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
...TAGG700.034.0000043
..CTGAG252.0292943E-434.0000044
T...CCC553.274181E-1134.0000042
T...CAC850.034.0000042
AT...TC553.274181E-1134.0000041
T...ACT252.0292943E-434.0000042
T...AAT1000.034.0000042
T...AAG700.034.0000042
AT...GA1000.034.0000041
GA...GG351.092525E-634.0000041
GA...GA252.0292943E-434.0000041
GA...AA700.034.0000041
..GATTT351.092525E-634.0000044
..TAGTC252.0292943E-434.0000044
..GATGC252.0292943E-434.0000044
..CTATC252.0292943E-434.0000044
..TAGGA252.0292943E-434.0000044
GG...AG252.0292943E-434.0000041
..TGTTA252.0292943E-434.0000044
.GAAGGG351.092525E-634.0000045
Produced by FastQC (version 0.11.5)