FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36350676
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACT21650.09.96093732
CGTCGCG7600.09.83968111
AGTCGAC23300.09.25557331
GTCGCGC8350.08.95586512
GAGGTAA115000.08.783371
AGGTAAT124550.08.6356882
GAGGTAG94200.08.4801221
AGGTAGA115000.07.8227112
CAGGTAA198800.07.8151021
GGGGTAA60950.07.72656441
AGGTAAC100000.07.5884122
TCGACTC29100.07.56205133
AGGTAAA183700.07.53135822
CAGGTAG122500.07.13182641
GGTTACC51950.07.11655951
AGGTAGT75900.07.0999642
GAAGTAA136100.06.96891981
GGTAATT88100.06.81599573
CGGGTAA18150.06.7898121
CGGGTCG7506.0954335E-96.7485881
Produced by FastQC (version 0.11.5)