FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068727
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences14660870
Sequences flagged as poor quality0
Sequence length40
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................352490.24042911505251732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCA..250.00830905727.24
.ATGGAA250.00830905727.21
.AAAAAA553.6601945E-521.6363641
TAAA...400.003157409821.251
CTGG...504.7871072E-420.4000022
GCTG...651.3221877E-418.3076933
TGTT...600.001629640717.0000023
TTAA...700.00455500514.5714282
CTGC...700.00455500514.5714283
CGG....950.002355162312.5263161
ACA....1852.228171E-711.9459471
G....C.1157.299299E-411.8260876
TAT....1802.0743028E-611.3333341
.....CA1200.001042825311.33333420
CG.....3152.3646862E-1110.7936511
AG.....9700.010.6907221
CA.....11000.010.6636361
TCC....2405.178299E-810.6250014
TG.....10850.010.3410131
AA.....11350.010.1850211