FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068743
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21188227
Sequences flagged as poor quality0
Sequence length95
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN267420212.621169293683703No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNN530580.2504126466079489No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNN525870.24818971403317514No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNN348520.16448757132911593No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNN322700.15230155878545193No Hit
NNNNNNNNNNNNTNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNN230560.10881514531631174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG4252.6252674E-65.222975388-89
Produced by FastQC (version 0.11.5)