FastQCFastQC Report
Sat 23 Apr 2022
EGAF00000068787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000068787
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences16019305
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
........................................334240.2086482528424298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAC.200.00279005834.03
CAATA..200.00279005834.03
A..C..C200.00279005834.015
A....T.305.909017E-428.33333210
.A...G.250.0083090827.19999914
TA..T..250.0083090827.1999996
T....A.250.0083090827.19999910
ACATC..250.0083090827.1999993
TT..C..250.0083090827.1999991
..CACCA250.0083090827.19999926
A....CT250.0083090827.19999921
CCCGTC.250.0083090827.19999915
CATGT..250.0083090827.1999996
...CATT250.0083090827.19999925
.A....T457.743511E-626.4444479
G....T.452.3491206E-422.66666810
.AAAAAA452.3491206E-422.6666681
...C..A504.7871345E-420.412
..T..C.504.7871345E-420.416
A..C...607.1532915E-519.8333327
Produced by FastQC (version 0.11.5)