FastQCFastQC Report
Mon 20 May 2024
EGAF00000103202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000103202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180875692
Total Bases18 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC15271300.8442980829065743TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2598210.143646167778034TruSeq Adapter, Index 8 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)