FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7804
Sequences flagged as poor quality0
Sequence length52-162
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT351645.053818554587394No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG177722.7703741670938No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG4275.471553049718094No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACCTGTGGTAGTT3043.8954382368016405No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT2132.7293695540748333No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC2012.5756022552537163No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAACTTGTGGTAGTTGG1592.037416709379805No Hit
CGTGTCTCTAAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT921.1788826242952333No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCT660.8457201435161457No Hit
CGTGTCTCTATTGTTGGATCATAATTCGTCCACAAAATGATTCTGAATTA390.49974372116863147No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTTA360.4613018964633521No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAAACCTGTGGTAGTTG320.4100461301896463No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATAGACTGAATATAAACTTGTGGTAG290.371604305484367No Hit
CGTGTCTCTAAGGCCTGCTGAATGACTGAATATAACTTGTGGTAGTTGGA290.371604305484367No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTGTGGTAGTTG200.2562788313685289No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAACCTGTGGTAGT190.24346488980010253No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG180.23065094823167606No Hit
CGTGTCTCTAAGGCCTGCTGAAATAGACTGAATATAACTTGTGGTAGTTG170.21783700666324962No Hit
CGTGTCTCTAAGGCCTGCTGAAAATAGACTGAATATAAACTTAGTGGTAG170.21783700666324962No Hit
CGTGTCTCTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG160.20502306509482315No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGG150.19220912352639674No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATAATAAACTTGTGGTAGT150.19220912352639674No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAACCTGTGGTAGTTGG140.17939518195797027No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGGATTAG140.17939518195797027No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA140.17939518195797027No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAATGATTCTGAATTTAG130.1665812403895438No Hit
CGTGTCTCTAAGGCCTGCTGAAATAGACTGAATATAAACTTGTGGTAGTT130.1665812403895438No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGCAGTT110.14095335725269093No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAGACTTGTGGTAGTT110.14095335725269093No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCCCAAAATGATTCTGAATTAG110.14095335725269093No Hit
CGTGTCTCTAAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT110.14095335725269093No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCAACAAAATGATTCTGAATTA100.12813941568426446No Hit
CGTGTCTCTAAGGCCTGCTGACAATGACTGAATATAAACTTGTGGTAGTT100.12813941568426446No Hit
CGTGTCTCTAAGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG100.12813941568426446No Hit
CGTGTCTCTAAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT100.12813941568426446No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAATGACTCTGAATTAG100.12813941568426446No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAATGATCCTGAATTAG100.12813941568426446No Hit
CGTGTCTCTAAGGCCTGCTGAACATGACTGAATATAAACTTGTGGTAGTT90.11532547411583803No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAATAGATTCTGAATTAGC90.11532547411583803No Hit
CGTGTCTCTATTGTTGGATCATATCGTCCACAAAATGATTCTGAATTAGC80.10251153254741158No Hit
CGTGTCTCTATTGTTGGATCATAATTCGTCCACAAAAATGATTCTGAATT80.10251153254741158No Hit
CGTGTCTCTAAGGCCTGCTGAAAATAGACTGAATATAAACCTGTGGTAGT80.10251153254741158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)