FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17941
Sequences flagged as poor quality0
Sequence length50-345
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT776243.264032105233824No Hit
CGTGTCTCTAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT475626.509113204392175No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG15418.589264812440778No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACCTGTGGTAGTT13357.441056797279972No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT2501.3934563290786466No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAAACCTGTGGTAGTTG2331.2987012987012987No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTGTGGTAGTTG1490.8304999721308733No Hit
CGTGTCTCTAGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT1470.8193523214982443No Hit
CGTGTCTCTAAGGCCTGCTGAAAATAGATGAATATAAACTTGTGGTAGTT1050.5852516582130316No Hit
CGTGTCTCTAAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT680.3790201215093919No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGG600.33442951897887524No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAACTTGTGGTAGTTGG480.26754361518310016No Hit
CGTGTCTCTAAGGCCTGCTGAAAATAGACTGAATATAAACTTAGTGGTAG460.256395964550471No Hit
CGTGTCTCTAGAATGGTCCTGCAACCAGTAATATGCATATTAAAACAAGA430.2396744886015272No Hit
CGTGTCTCTAGAATGGTCCTGCACCAGTAATATGCATATTAAAAACAAGA410.22852683796889803No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAACCTGTGGTAGT390.21737918733626885No Hit
CGTGTCTCTAAGGCCTGCTGAAAATAGATGAATATAAACTTAGTGGTAGT290.16164093417312303No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGATGAATATAAACTTGTGGTAGTTG290.16164093417312303No Hit
CGTGTCTCTAAGGCCTGCTGAATGACTGAATATAACTTGTGGTAGTTGGA260.14491945822417926No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGCAGTT250.13934563290786467No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAAACTGTGGTAGTTGG240.13377180759155008No Hit
CGTGTCTCTAAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT240.13377180759155008No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAGACTTGTGGTAGTT230.1281979822752355No Hit
CGTGTCTCTAGAATGGTCCTGCACCAGTAATATGCATATTAAGACAAGAT220.1226241569589209No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAAGT200.11147650632629172No Hit
CGTGTCTCTAAGGCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG190.10590268100997716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)