FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14720
Sequences flagged as poor quality0
Sequence length49-446
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGCACTCAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT773152.52038043478261No Hit
AGACGCACTCGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT315921.460597826086957No Hit
AGACGCACTCAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG224815.271739130434783No Hit
AGACGCACTCAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT1521.0326086956521738No Hit
AGACGCACTCAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG1390.9442934782608695No Hit
AGACGCACTCGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT1030.6997282608695653No Hit
AGACGCACTCAGGCCTGCTAGAAAATGACTGAATATAAACTTGTGGTAGT900.6114130434782609No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGG660.4483695652173913No Hit
AGACGCACTCGAATGGTCCTGCAACCAGTAATATGCATATTAAAACAAGA400.2717391304347826No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGAATATAAACTTGTAGGTAGT380.25815217391304346No Hit
AGACGCACTCAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT220.14945652173913043No Hit
AGACGCACTCGAATGGTCCTGCACTAGTAATATGCATATTAAAACAAGAT220.14945652173913043No Hit
AGACGCACTCAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGG200.1358695652173913No Hit
AGACGCACTCGAATGGTCCTGCACCAGTAATATGCATATTAAAAACAAGA170.11548913043478261No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG170.11548913043478261No Hit
AGACGCACTCAGGCCTGCTGAAAATGCCTGAATATAAACTTGTGGTAGTT170.11548913043478261No Hit
AGACGCACTCAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT160.10869565217391304No Hit
AGACGCACTCAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT160.10869565217391304No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGAATATAAACTTGCGGTAGTT150.10190217391304347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)