FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7780
Sequences flagged as poor quality0
Sequence length59-201
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG362846.63239074550128No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT155620.0No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT104813.470437017994858No Hit
ACGAGTGCGTTTGTTGGATCATATTCGCCCACAAAATGATTCTGAATTAG4816.182519280205655No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG1101.4138817480719794No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGCT781.0025706940874035No Hit
ACGAGTGCGTTTGTTGGATCATATTCGCCCACAAAATAGATTCTGAATTA750.9640102827763496No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA650.8354755784061696No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC480.6169665809768637No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC400.5141388174807198No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATAATAAACTTGTGGTAG320.4113110539845758No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATAGACTGAATATAAACTTGTGGTAG230.29562982005141386No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAATAGATTCTGAATTA210.2699228791773779No Hit
ACGAGTGCGTTTGTTGGCTCATATTCGTCCACAAAATGATTCTGAATTAG170.21850899742930593No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTTCACAAAATGATTCTGAATTAG160.2056555269922879No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAAATAGATTCTGAATTAGC140.17994858611825193No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAATGATTCTGAGTTAG140.17994858611825193No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTAGTGGTAG130.16709511568123392No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT110.14138817480719795No Hit
ACGAGTGCGTTTGTTGGATCATATTTCGCCCACAAAATGATTCTGAATTA110.14138817480719795No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCATAAAATGATTCTGAATTAG100.12853470437017994No Hit
ACGAGTGCGTTTGTTGGATCATATTACGTCCACAAAATGATTCTGAATTA80.10282776349614395No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTAGTAGT80.10282776349614395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)