FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9430
Sequences flagged as poor quality0
Sequence length53-255
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT473450.201484623541894No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT186419.766702014846235No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT154316.3626723223754No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT3223.414634146341464No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG971.0286320254506893No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAAAACAAGA480.5090137857900319No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAACAAGATTT410.43478260869565216No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATAGACTGAATATAAACTTGTGGTAG410.43478260869565216No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT270.28632025450689286No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG260.2757158006362672No Hit
ACGAGTGCGTAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAG210.2226935312831389No Hit
ACGAGTGCGTGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGAT180.19088016967126192No Hit
ACGAGTGCGTGAATGGGTCCTGCACCAGTAATATGCATATTAAAACAAGA170.18027571580063625No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAAGT160.1696712619300106No Hit
ACGAGTGCGTGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT160.1696712619300106No Hit
ACGAGTGCGTGAATGGTTCTGCACCAGTAATATGCATATTAAAACAAGAT150.15906680805938495No Hit
ACGAGTGCGTGAATGGTCCTGCACTAGTAATATGCATATTAAAACAAGAT140.14846235418875928No Hit
ACGAGTGCGTGAATGGTCCTGCACAGTAATATGCATATTAAAACAAGATT140.14846235418875928No Hit
ACGAGTGCGTAGGCCTGCTGAACATGACTGAATATAAACTTGTGGTAGTT130.1378579003181336No Hit
ACGAGTGCGTGAATGGTCCTGTACCAGTAATATGCATATTAAAACAAGAT130.1378579003181336No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAAG120.12725344644750797No Hit
ACGAGTGCGTAGGCCTGTTGAAAATGACTGAATATAAACTTGTGGTAGTT110.11664899257688228No Hit
ACGAGTGCGTGAATCGTCCTGCACCAGTAATATGCATATTAAAACAAGAT100.10604453870625664No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCGTATTAAAACAAGAT100.10604453870625664No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGATTGAATATAAACTTGTGGTAGT100.10604453870625664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)