FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21545
Sequences flagged as poor quality0
Sequence length49-489
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT953444.25156648874449No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA887741.20213506614064No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGTTGTCAGTCATTTTCAGCAGGCCTACGCACTCGT4332.0097470410768157No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGACAACCTTGGGAGTATGTCAGGGTACGCACTCGT2140.9932699002088652No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAAAGTAAAGTCCCCATATTA1850.8586679043861685No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGTTGTCAGTCATTTCAGCAGGCCTACGCACTCGT1520.7055001160362032No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG1370.6358783940589464No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT1050.4873520538407983No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT950.44093757252262705No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGACAACCTTGGGAGTATGTCAGGGTACGCACTCGT630.292411232304479No Hit
ACGAGTGCGTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGT350.16245068461359943No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAAGTAAAGTTCCCATATTAA340.15780923648178233No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAGTATAAACTTGTGGTAGTT340.15780923648178233No Hit
ACGAGTGCGTAGGCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG330.1531677883499652No Hit
ACGAGTGCGTAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT330.1531677883499652No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGAAGTAAAGTTCCCATATTA280.12996054769087956No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAAGGTAAAGTTCCCATATTA260.1206776514272453No Hit
ACGAGTGCGTACCTTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA260.1206776514272453No Hit
ACGAGTGCGTACCCTGACATATTCCCAAGGAAAGTAAAGTTCCCATATTA250.11603620329542817No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAGAGTAAAGTTCCCATATTA250.11603620329542817No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCACATTA240.11139475516361104No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGTTGTACAGTCATTTTCAGCAGGCCTACGCACTCGT240.11139475516361104No Hit
ACGAGTGCGTAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT240.11139475516361104No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGAAAGTAAAGTTCCCATATT230.10675330703179392No Hit
ACGAGTGCGTAGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG230.10675330703179392No Hit
ACGAGTGCGTATCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA220.10211185889997679No Hit
ACGAGTGCGTACCCTGACATACTCCCAGGGAAAGTAAAGTTCCCATATTA220.10211185889997679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)