FastQCFastQC Report
Tue 7 May 2024
EGAF00000127533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18781
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length48-345
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATGCGAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT961751.206006069964324No Hit
TCTCTATGCGGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT491726.180714551940792No Hit
TCTCTATGCGAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG217411.57552845961344No Hit
TCTCTATGCGAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT2741.4589212501996698No Hit
TCTCTATGCGAGGCCTGCTAGAAAATGACTGAATATAAACTTGTGGTAGT2041.0862041424844258No Hit
TCTCTATGCGAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG1280.681539854107875No Hit
TCTCTATGCGGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT1210.6442681433363505No Hit
TCTCTATGCGGAATGGTCCTGCAACCAGTAATATGCATATTAAAACAAGA620.33012086683350195No Hit
TCTCTATGCGAGGCCTGCTGAAAATGACTGAATATAAGCTTGTGGTAGTT580.30882274639263085No Hit
TCTCTATGCGAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT320.17038496352696875No Hit
TCTCTATGCGGAATGGTCCTGCACCAGTAATATGCATATTAAAAACAAGA310.16506043341675097No Hit
TCTCTATGCGAGGCCTGTTGAAAATGACTGAATATAAACTTGTGGTAGTT300.1597359033065332No Hit
TCTCTATGCGCGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT290.15441137319631543No Hit
TCTCTATGCGAGGCCTGCTGAAAATGACTGAATATAAACTTGTAGGTAGT280.14908684308609765No Hit
TCTCTATGCGAGGCCTGCTAGAAATGACTGAATATAAACTTGTGGTAGTT270.14376231297587988No Hit
TCTCTATGCGAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT260.1384377828656621No Hit
TCTCTATGCGAGGCCTGCTGCAAATGACTGAATATAAACTTGTGGTAGTT230.12246419253500879No Hit
TCTCTATGCGAGGCCTGCTGAAATGACTGAATATAAGCTTGTGGTAGTTG230.12246419253500879No Hit
TCTCTATGCGAGGCCTGCTGAAAATGACTGAATATAAACTTGCGGTAGTT220.11713966242479101No Hit
TCTCTATGCGAGGCCTGCTGAACATGACTGAATATAAACTTGTGGTAGTT190.10116607209413768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)