FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25044
Sequences flagged as poor quality0
Sequence length46-551
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATGCGAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT1098443.858808497045196No Hit
TCTCTATGCGACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA1027641.03178406005431No Hit
TCTCTATGCGAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG4481.7888516211467815No Hit
TCTCTATGCGAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT4351.736942980354576No Hit
TCTCTATGCGACCCTGACATAACTCCCAAGGAAAGTAAAGTTCCCATATT2731.0900814566363202No Hit
TCTCTATGCGAGGCCTGCTAGAAAATGACTGAATATAAACTTGTGGTAGT2350.9383485066283342No Hit
TCTCTATGCGACCCTGACATACTACCCAAGGAAAGTAAAGTTCCCATATT1110.4432199329180642No Hit
TCTCTATGCGAGGCCTGCTGAAAATGACTGAATATAAGCTTGTGGTAGTT790.31544481712186556No Hit
TCTCTATGCGACCCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATT700.27950806580418464No Hit
TCTCTATGCGACCCTGATATACTCCCAAGGAAAGTAAAGTTCCCATATTA480.19166267369429804No Hit
TCTCTATGCGACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATCA470.18766970132566682No Hit
TCTCTATGCGAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG440.1756907842197732No Hit
TCTCTATGCGACCCTGACATACTCCCAAGGAGAGTAAAGTTCCCATATTA430.171697811851142No Hit
TCTCTATGCGAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT420.16770483948251078No Hit
TCTCTATGCGAGGCCTGCTAGAAAAATGACTGAATATAAACTTGTGGTAG410.16371186711387958No Hit
TCTCTATGCGACCCTGACATACTCCCAGGGAAAGTAAAGTTCCCATATTA370.14773997763935476No Hit
TCTCTATGCGACCCTGACATACTCCTAAGGAAAGTAAAGTTCCCATATTA340.13576106053346113No Hit
TCTCTATGCGAGGCCTGCTAGAAAATGACTGAATAATAAACTTGTGGTAG330.1317680881648299No Hit
TCTCTATGCGACCCTGACATACTCCCAAGGAAAGTAAAAGTTCCCATATT320.12777511579619868No Hit
TCTCTATGCGAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT310.12378214342756748No Hit
TCTCTATGCGAGGCCTGCTGAACATGACTGAATATAAACTTGTGGTAGTT290.11579619869030507No Hit
TCTCTATGCGACCCTGACATATTCCCAAGGAAAGTAAAGTTCCCATATTA280.11180322632167385No Hit
TCTCTATGCGAGGCCTGCTGAAAATGATTGAATATAAACTTGTGGTAGTT270.10781025395304264No Hit
TCTCTATGCGACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATACTA270.10781025395304264No Hit
TCTCTATGCGAGGCCTGCTGAAAATGACTGAATATAAAACTTGTGGTAGT260.10381728158441145No Hit
TCTCTATGCGAGGCCTGTTGAAAATGACTGAATATAAACTTGTGGTAGTT260.10381728158441145No Hit
TCTCTATGCGACCCTGACATACTCTCAAGGAAAGTAAAGTTCCCATATTA260.10381728158441145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)