FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9552
Sequences flagged as poor quality0
Sequence length49-202
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTATCAGCAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT398041.66666666666667No Hit
TAGTATCAGCAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG214222.42462311557789No Hit
TAGTATCAGCTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG167817.567001675041876No Hit
TAGTATCAGCTTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC2072.1670854271356785No Hit
TAGTATCAGCAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG1801.8844221105527637No Hit
TAGTATCAGCAGGCCTGCTGAATGACTGAATATAACTTGTGGTAGTTGGA1071.1201842546063652No Hit
TAGTATCAGCAGGCCTGCTGAAATGACTGATGAGGTGGACGAATATGATCCAACAAGCTGATACTA920.9631490787269681No Hit
TAGTATCAGCAGGCCTGCTGAAAATGACTGATGAGGTGGACGAATATGATCCAACAAGCTGATACTA690.7223618090452262No Hit
TAGTATCAGCTTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCT690.7223618090452262No Hit
TAGTATCAGCAGGCCTGCTGAAATGACTGGACGAATATGATCCAACAAGCTGATACTA650.6804857621440537No Hit
TAGTATCAGCTTGTTGGATCATATTCGTCCACCTCATCAGTCATTTTCAGCAGGCCTGCTGATACTA540.5653266331658292No Hit
TAGTATCAGCAGGCCTGCTGAAAATGACTGGACGAATATGATCCAACAAGCTGATACTA530.554857621440536No Hit
TAGTATCAGCAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT480.5025125628140703No Hit
TAGTATCAGCAGGCCTGCTAGAAAATGACTGAATATAAACTTGTGGTAGT430.4501675041876047No Hit
TAGTATCAGCTTGTTGGATCATATTCGTCCAGTCATTTTCAGCAGGCCTGCTGATACTA400.41876046901172526No Hit
TAGTATCAGCAGGCCTGCTGAATGACTGGACGAATATGATCCAACAAGCTGATACTA240.25125628140703515No Hit
TAGTATCAGCTTGTTGGATCATAATTCGTCCACAAAATGATTCTGAATTA210.2198492462311558No Hit
TAGTATCAGCAGGCCTGCTGAAATAGACTGAATATAAACTTGTGGTAGTT190.19891122278056952No Hit
TAGTATCAGCAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG190.19891122278056952No Hit
TAGTATCAGCAGGCCTGCTAGAAATGACTGAATATAAACTTGTGGTAGTT180.18844221105527637No Hit
TAGTATCAGCTTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA180.18844221105527637No Hit
TAGTATCAGCAGGCCTGCTGAATGACTGATGAGGTGGACGAATATGATCCAACAAGCTGATACTA170.17797319932998323No Hit
TAGTATCAGCAGGCCTGCTGAATAGACTGAATATAACTTGTGGTAGTTGG160.16750418760469013No Hit
TAGTATCAGCAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT140.14656616415410384No Hit
TAGTATCAGCTTGTTGGATCATATTCGTCCAACAAAATGATTCTGAATTA130.1360971524288107No Hit
TAGTATCAGCAGGCCTGCTGCAAATGACTGAATATAAACTTGTGGTAGTT130.1360971524288107No Hit
TAGTATCAGCAGGCCTGCTGACAATGACTGAATATAAACTTGTGGTAGTT130.1360971524288107No Hit
TAGTATCAGCAGGCCTGCTGAAATGACTGGCGAATATGATCCAACAAGCTGATACTA100.10469011725293131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)