FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9033
Sequences flagged as poor quality0
Sequence length59-192
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCGTGTCTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG391643.35215321598583No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT255228.251965017159304No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC5265.823093103066534No Hit
CTCGCGTGTCAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT4284.738182220746153No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCT2512.778700321045057No Hit
CTCGCGTGTCAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG991.0959814015277316No Hit
CTCGCGTGTCAGGCCTGCTGAAAAAATGACTGAATATAAACTTGTGGTAG830.9188530942101184No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCACAATAGATTCTGAATTAGC640.7085132292704528No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGAATATAAACTTAGTGGTAG250.2767629801837706No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCATAAAATGATTCTGAATTAG230.25462194176906894No Hit
CTCGCGTGTCAGGCCTGCTAGAAAAATGACTGAATATAAACTTGTGGTAG220.24355142256171813No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGCCTAGGTGGACGAATATGATCCAACAAGACACGCGAG200.2214103841470165No Hit
CTCGCGTGTCAGGCCTGCTGAAAATGACTGCCTAGGTGGACGAATATGATCCAACAAGACACGCGAG190.21033986493966564No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA180.19926934573231483No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCAACAAAATGATTCTGAATTA170.18819882652496403No Hit
CTCGCGTGTCTTGTTGATCATATTCGTCCACAAAATGATTCTGAATTAGC170.18819882652496403No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG170.18819882652496403No Hit
CTCGCGTGTCTTGTTGGATCATATCGTCCACAAAATGATTCTGAATTAGC170.18819882652496403No Hit
CTCGCGTGTCTTGTTGGATTATATTCGTCCACAAAATGATTCTGAATTAG160.1771283073176132No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCACCTAGGCAGTCATTTTTCAGCAGGCCTGACACGCGAG160.1771283073176132No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCACAAATAGATTCTGAATTAG150.16605778811026237No Hit
CTCGCGTGTCTTGTTGGATCATATTTGTCCACAAAATGATTCTGAATTAG150.16605778811026237No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCCCAAAAATGATTCTGAATTA150.16605778811026237No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGC150.16605778811026237No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGAATATAAACTTGGCGGTAG140.15498726890291156No Hit
CTCGCGTGTCTTGTTGGATCATATTCGTCCACCTAGGCAGTCATTTTCAGCAGGCCTGACACGCGAG140.15498726890291156No Hit
CTCGCGTGTCTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC130.14391674969556073No Hit
CTCGCGTGTCTTGTTGGATATATTCGTCCACAAAATGATTCTGAATTAGC130.14391674969556073No Hit
CTCGCGTGTCTTGTTGGATCCTATTCGTCCACAAAATGATTCTGAATTAG130.14391674969556073No Hit
CTCGCGTGTCTTGTTGGATCATATACGTCCACAAAATGATTCTGAATTAG120.1328462304882099No Hit
CTCGCGTGTCTTGTTGGATCAATATTCGTCCACAAAATGATTCTGAATTA100.11070519207350825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)