FastQCFastQC Report
Tue 7 May 2024
EGAF00000127543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12071
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length55-206
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG520043.078452489437495No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT271522.49192279015823No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT159613.221771187142739No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC5324.407257062380912No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCT2221.8391185485875237No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG2131.7645596885096513No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA1180.977549498798774No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATAATAAACTTGTGGTAG550.4556374782536658No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAATAGATTCTGAATTAGC520.43078452489437496No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCCCAAAAATGATTCTGAATTA420.34794134703007207No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATAGACTGAATATAAACTTGTGGTAG380.31480407588435094No Hit
CGTGTCTCTATTGTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC380.31480407588435094No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCAACAAAATGATTCTGAATTA370.30651975809792065No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG320.26509816916576917No Hit
CGTGTCTCTATTGTTGGCTCATATTCGTCCACAAAATGATTCTGAATTAG280.23196089802004805No Hit
CGTGTCTCTATTGTTGGATTATATTCGTCCACAAAATGATTCTGAATTAG230.1905393090878966No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCCCAAAATGATTCTGAATTAG220.1822549913014663No Hit
CGTGTCTCTATTGTTGGATCCTATTCGTCCACAAAATGATTCTGAATTAG210.17397067351503603No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAACTTAGTGGTAG200.16568635572860577No Hit
CGTGTCTCTATTGTTGGATCATATCGTCCACAAAATGATTCTGAATTAGC200.16568635572860577No Hit
CGTGTCTCTAAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAG200.16568635572860577No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAGATGATTCTGAATTAG180.14911772015574518No Hit
CGTGTCTCTAAGGCCTGCTGGAAATGACTGAATATAAACTTGTGGTAGTT180.14911772015574518No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCTACAAAATGATTCTGAATTAG170.14083340236931488No Hit
CGTGTCTCTATGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC170.14083340236931488No Hit
CGTGTCTCTATTGTTGGATCATATTGTCCACAAAATGATTCTGAATTAGC170.14083340236931488No Hit
CGTGTCTCTATTGTTGGATCATATTCGTTCACAAAATGATTCTGAATTAG160.13254908458288459No Hit
CGTGTCTCTATTGTTGATCATATTCGTCCACAAAATGATTCTGAATTAGC160.13254908458288459No Hit
CGTGTCTCTATTTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC160.13254908458288459No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCACAAAATAGATTCTGAATTA160.13254908458288459No Hit
CGTGTCTCTATTGTTGGATCATATTCGTCCATAAAATGATTCTGAATTAG160.13254908458288459No Hit
CGTGTCTCTATTGTTGGATCATATTTGTCCACAAAATGATTCTGAATTAG150.12426476679645432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)