FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10241
Sequences flagged as poor quality0
Sequence length53-379
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT480846.94854018162289No Hit
CGTGTCTCTAACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA457644.683136412459724No Hit
CGTGTCTCTAAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG670.6542329850600527No Hit
CGTGTCTCTAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT610.5956449565472122No Hit
CGTGTCTCTAAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT460.4491748852651108No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTAGTAGTT320.3124694854018162No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTGGTT240.2343521140513622No Hit
CGTGTCTCTAAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT230.2245874426325554No Hit
CGTGTCTCTAACCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTAA210.2050580997949419No Hit
CGTGTCTCTAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGCT170.16599941411971486No Hit
CGTGTCTCTAACCCTGACATACTCCCAAGGAAAGTAAGGTTCCCATATTA150.14647007128210135No Hit
CGTGTCTCTACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTAA120.1171760570256811No Hit
CGTGTCTCTAACCCTGACATACTCCAAGGAAAGTAAAGTTCCCATATTAA120.1171760570256811No Hit
CGTGTCTCTAAGGCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG110.10741138560687433No Hit
CGTGTCTCTAAGGCCTGTTGAAAATGACTGAATATAAACTTGTGGTAGTT110.10741138560687433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)