FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14605
Sequences flagged as poor quality0
Sequence length43-176
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGCACTCTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG607841.615884970900375No Hit
AGACGCACTCAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT362924.84765491270113No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC6584.505306401917151No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT5994.1013351591920575No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCAGTCATTTTCAGCAGGCCTGAGTGCGTCT5163.5330366312906536No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCT3042.0814789455665865No Hit
AGACGCACTCAGGCCTGCTGAAAAATGACTGGACGAATATGATCCAACAAGAGTGCGTCT2021.3830879835672714No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGGACGAATATGATCCAACAAGAGTGCGTCT1390.9517288599794591No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCAGTACATTTTCAGCAGGCCTGAGTGCGTCT1340.9174940089010613No Hit
AGACGCACTCAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG1200.8216364258815475No Hit
AGACGCACTCAGGCCTGCTGAAATGACTGGACGAATATGATCCAACAAGAGTGCGTCT770.5272167066073262No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCAGTCATTTCAGCAGGCCTGAGTGCGTCT740.5066757959602876No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCAACAAAATGATTCTGAATTA610.41766518315645323No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA610.41766518315645323No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCATCAGTCATTTTCAGCAGGCCTGAGTGCGTCT530.36288942143101677No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCACAATAGATTCTGAATTAGC520.35604245121533723No Hit
AGACGCACTCAGGCCTGCTGAAAAAATGACTGAATATAAACTTGTGGTAG500.3423485107839781No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCAGTCATTTTTCAGCAGGCCTGAGTGCGTCT470.3218076001369394No Hit
AGACGCACTCAGGCCTGCTAGAAAAATGACTGAATATAAACTTGTGGTAG290.1985621362547073No Hit
AGACGCACTCAGGCCTGCTGAAAAATGACTGAATATAAACTTAGTGGTAG240.16432728517630948No Hit
AGACGCACTCTTGTTGGATCATATTCGTTCACAAAATGATTCTGAATTAG230.15748031496062992No Hit
AGACGCACTCTTGTTGATCATATTCGTTCAGTCATTTTCAGCAGGCCTGAGTGCGTCT220.15063334474495035No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCATCAGTCATTTTTCAGCAGGCCTGAGTGCGTCT220.15063334474495035No Hit
AGACGCACTCAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG220.15063334474495035No Hit
AGACGCACTCTTGTTGGATCATATTTGTCCACAAAATGATTCTGAATTAG220.15063334474495035No Hit
AGACGCACTCTTGTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC210.1437863745292708No Hit
AGACGCACTCTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC200.13693940431359122No Hit
AGACGCACTCTTGTTGGATTATATTCGTCCACAAAATGATTCTGAATTAG190.13009243409791166No Hit
AGACGCACTCAGGCCTGCTGAAAAATGACTGATGGACGAATATGATCCAACAAGAGTGCGTCT190.13009243409791166No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCACCATCAGTCATTTTTCAGCAGGCCTGAGTGCGTCT170.11639849366655255No Hit
AGACGCACTCTTGTTGGATCAATATTCGTCCACAAAATGATTCTGAATTA160.10955152345087299No Hit
AGACGCACTCTTGTTGGATCATATTCGTCCAGTCATTTTTCAGCAGGGCCTGAGTGCGTCT150.10270455323519344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)