FastQCFastQC Report
Tue 7 May 2024
EGAF00000127549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10926
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length53-203
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG452141.3783635365184No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT383235.07230459454512No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC3613.304045396302398No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA3463.1667581914698886No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG2832.590151931173348No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT2492.2789676002196595No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG1341.226432363170419No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAATAGATTCTGAATTAG860.7871133077063883No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAATAGATTCTGAATTA380.3477942522423577No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC320.29287937030935385No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAAACTTGTGGTAGT290.2654219293428519No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGAATATAACTTGTGGTAGTTGGA270.2471169686985173No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT250.2288120080541827No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAATAGATTCTGAATTAGCT200.18304960644334614No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTTCACAAAATGATTCTGAATTAG200.18304960644334614No Hit
ACGAGTGCGTTTGTTGGCTCATATTCGTCCACAAAATGATTCTGAATTAG160.14643968515467692No Hit
ACGAGTGCGTTTTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATT150.1372872048325096No Hit
ACGAGTGCGTTTGTTGGATCATATTTGTCCACAAAATGATTCTGAATTAG150.1372872048325096No Hit
ACGAGTGCGTTTGTTGGATCCTATTCGTCCACAAAATGATTCTGAATTAG150.1372872048325096No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCCCAAAATGATTCTGAATTAG140.1281347245103423No Hit
ACGAGTGCGTAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT130.118982244188175No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCTG130.118982244188175No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCATAAAATGATTCTGAATTAG120.10982976386600769No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG120.10982976386600769No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTAGTGGTAG120.10982976386600769No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCGCAAAATGATTCTGAATTAG120.10982976386600769No Hit
ACGAGTGCGTAGGCCTGCTGACAATGACTGAATATAAACTTGTGGTAGTT110.10067728354384037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)