FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20894
Sequences flagged as poor quality0
Sequence length47-445
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGCACTCACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA941945.07992725184263No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT771136.905331674164835No Hit
AGACGCACTCAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT6162.9482147985067484No Hit
AGACGCACTCAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG2091.0002871637790753No Hit
AGACGCACTCACCCTGACATACTCCCAAGGCACTTTTTGGAAAAGCCAGT1850.8854216521489423No Hit
AGACGCACTCAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG1790.8567052742414091No Hit
AGACGCACTCACCCTGACATACTCCCAAGGAAAGTAAAAGTTCCCATATT1680.8040585814109314No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGGCTTTTCCAAAAAGTGCCTT1290.6174021250119651No Hit
AGACGCACTCACCCTGACATACTCCCAAGGCACTTTTTGGAAAGCCAGTCATTTTCAGCAGGCCTGAGTGCGTCT1130.5408251172585431No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGAATATAAAACTTGTGGTAGT990.47382023547429886No Hit
AGACGCACTCACCCTGACATACTCCCAAGGCAGGTTCAGTTAGTCTTCCA920.4403177945821767No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGGCCACTTTGTGCTCTTGAGC650.311094093998277No Hit
AGACGCACTCAGGCCTGCTAGAAAATGACTGAATAATAAACTTGTGGTAG540.2584474011677994No Hit
AGACGCACTCACCCTGACATACTCCCAAGGCACTTTTGGAAAGCCAGTCATTTTCAGCAGGCCTGAGTGCGTCT400.19144251938355508No Hit
AGACGCACTCACCCTGACATACTTCCAAGGAAAGTAAAGTTCCCATATTA380.18187039341437733No Hit
AGACGCACTCACCCTGACATACTCCCAAGGAAGTAAAGTTCCCATATTAA370.17708433042978847No Hit
AGACGCACTCACCCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATT360.17229826744519958No Hit
AGACGCACTCACCCTGACATATTCCCAAGGAAAGTAAAGTTCCCATATTA320.15315401550684407No Hit
AGACGCACTCACCCTGACCTACTCCCAAGGAAAGTAAAGTTCCCATATTA310.1483679525222552No Hit
AGACGCACTCACTCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA310.1483679525222552No Hit
AGACGCACTCACCCTGATATACTCCCAAGGAAAGTAAAGTTCCCATATTA300.14358188953766632No Hit
AGACGCACTCATCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA290.13879582655307743No Hit
AGACGCACTCAGGCCTGCTAGAAAATGACTGAATATAAACTTGTGGTAGT270.1292237005838997No Hit
AGACGCACTCACCCTGTCATACTCCCAAGGAAAGTAAAGTTCCCATATTA250.11965157461472194No Hit
AGACGCACTCACCCTGACATACTCCTAAGGAAAGTAAAGTTCCCATATTA250.11965157461472194No Hit
AGACGCACTCAGGCCTGCTGAAAATGACTGGCTTTTCCAAAAGTGCCTTGGGAGTATGTCAGGGTGAGTGCGTCT250.11965157461472194No Hit
AGACGCACTCACCTTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA250.11965157461472194No Hit
AGACGCACTCACCCTGACATACTCTCAAGGAAAGTAAAGTTCCCATATTA230.11007944864554417No Hit
AGACGCACTCACCCTGACATACTCCCAAGGAAAGTAAAGTTACCCATATT220.10529338566095531No Hit
AGACGCACTCAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT210.10050732267636643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)