FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13777
Sequences flagged as poor quality0
Sequence length54-343
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCGTGTCGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT678149.21971401611381No Hit
CTCGCGTGTCAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT463533.643028235464904No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT4963.6002032372795236No Hit
CTCGCGTGTCAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG3582.5985337881977206No Hit
CTCGCGTGTCGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT2241.6258982361907526No Hit
CTCGCGTGTCAGGCCTGCTAGAAAATGACTGAATATAAACTTGTGGTAGT750.5443855701531538No Hit
CTCGCGTGTCAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG460.333889816360601No Hit
CTCGCGTGTCGAATGGTCCTGCACCAGTAATAATGCATATTAAAACAAGA450.32663134209189226No Hit
CTCGCGTGTCAGGCCTGCTAGAAAATGACTGAATAATAAACTTGTGGTAG370.26856354794222254No Hit
CTCGCGTGTCGAATGGTCCTGCACCAGTAATATGCATATTAAAAACAAGA360.2613050736735138No Hit
CTCGCGTGTCGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT280.20323727952384407No Hit
CTCGCGTGTCAGGCCTGCTAGAAAAATGACTGAATAATAAACTTGTGGTA280.20323727952384407No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGAATAATAAACTTGTGGTAG260.18872033098642665No Hit
CTCGCGTGTCGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGAT260.18872033098642665No Hit
CTCGCGTGTCAGGCCTGCTAGAAAAATGACTGAATATAAACTTGTGGTAG260.18872033098642665No Hit
CTCGCGTGTCAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG240.1742033824490092No Hit
CTCGCGTGTCGAATGGTCCTGCATCAGTAATATGCATATTAAAACAAGAT230.1669449081803005No Hit
CTCGCGTGTCGAATGGTCCTGCACTAGTAATATGCATATTAAAACAAGAT220.1596864339115918No Hit
CTCGCGTGTCAGGCCTGCTGAACATGACTGAATATAAACTTGTGGTAGTT210.15242795964288308No Hit
CTCGCGTGTCAGGCCTGCTGAAAATGACTAAATATAAACTTGTGGTAGTT210.15242795964288308No Hit
CTCGCGTGTCGAATGGTCCTGTACCAGTAATATGCATATTAAAACAAGAT200.14516948537417435No Hit
CTCGCGTGTCGAATGGTCCTGCACCAGTAATATGCATATTAAAACGAGAT200.14516948537417435No Hit
CTCGCGTGTCGAATGGTTCTGCACCAGTAATATGCATATTAAAACAAGAT190.13791101110546564No Hit
CTCGCGTGTCAGGCCTGCTGAAAATGACTGAATATAGACTTGTGGTAGTT190.13791101110546564No Hit
CTCGCGTGTCGATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATT160.11613558829933948No Hit
CTCGCGTGTCGAATGGTCCTGCACCAGTAATACGCATATTAAAACAAGAT160.11613558829933948No Hit
CTCGCGTGTCGAATGGTCCTGCACCCGTAATATGCATATTAAAACAAGAT150.10887711403063077No Hit
CTCGCGTGTCAGGCCTGTTGAAAATGACTGAATATAAACTTGTGGTAGTT140.10161863976192204No Hit
CTCGCGTGTCGAATGGTCTGCACCAGTAATATGCATATTAAAACAAGATT140.10161863976192204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)