FastQCFastQC Report
Tue 7 May 2024
EGAF00000127555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5168
Total Bases717.3 kbp
Sequences flagged as poor quality0
Sequence length52-534
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCTCGACATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG189136.5905572755418No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT183535.506965944272444No Hit
ACGCTCGACAAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG4248.204334365325078No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC2374.585913312693498No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCT791.5286377708978327No Hit
ACGCTCGACAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT701.3544891640866874No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAATAGATTCTGAATTAGC671.2964396284829722No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA470.9094427244582043No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAAATAGATTCTGAATTAG460.890092879256966No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATAATAAACTTGTGGTAGT280.541795665634675No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAAGATGATTCTGAATTAG170.3289473684210526No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAAACTTGTGGTAGT130.2515479876160991No Hit
ACGCTCGACAAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG110.21284829721362228No Hit
ACGCTCGACAAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT100.19349845201238391No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCAACAAAATGATTCTGAATTA100.19349845201238391No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAGACTTGTGGTAGTT80.15479876160990713No Hit
ACGCTCGACAAGGCCTGCTGACAATGACTGAATATAAACTTGTGGTAGTT70.13544891640866874No Hit
ACGCTCGACATTGTTGGATCATATTCGTCTACAAAATGATTCTGAATTAG70.13544891640866874No Hit
ACGCTCGACAAGGCCTGCTGAAAAATGACTGAATAATAAACTTGTGGTAG70.13544891640866874No Hit
ACGCTCGACATTGTTGGATCATATTTGTCCACAAAATGATTCTGAATTAG70.13544891640866874No Hit
ACGCTCGACAAGGCCTGCTGCAAATGACTGAATATAAACTTGTGGTAGTT70.13544891640866874No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGGATATAAACTTGTGGTAGTT60.11609907120743033No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAAAATGATTCTGAGTTAG60.11609907120743033No Hit
ACGCTCGACATTGTTGCATCATATTCGTCCACAAAATGATTCTGAATTAG60.11609907120743033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)