FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12449
Sequences flagged as poor quality0
Sequence length52-535
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT532542.77452004177042No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT429634.508795887219854No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT6655.341794521648325No Hit
ACGCTCGACAAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG6425.1570407261627444No Hit
ACGCTCGACAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT4433.5585187565266287No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAACAAGATTT1010.8113101453932042No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAAAAACAAGA730.5863924813238011No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAAAGCAAGAT560.44983532813880633No Hit
ACGCTCGACAGAATGGTCCTGCATCAGTAATATGCATATTAAAACAAGAT250.20081934291911No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAAGT230.18475379548558116No Hit
ACGCTCGACAGAATGGTCCTGCACTAGTAATATGCATATTAAAACAAGAT220.17672102176881677No Hit
ACGCTCGACAGAATGGTTCTGCACCAGTAATATGCATATTAAAACAAGAT210.16868824805205238No Hit
ACGCTCGACAGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGAT190.15262270061852357No Hit
ACGCTCGACAAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG190.15262270061852357No Hit
ACGCTCGACAGAATGGTCTGCACCAGTAATATGCATATTAAAACAAGATT170.13655715318499478No Hit
ACGCTCGACAAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG160.1285243794682304No Hit
ACGCTCGACAGAATGGTCCTGCACCAATAATATGCATATTAAAACAAGAT140.11245883203470158No Hit
ACGCTCGACAAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT140.11245883203470158No Hit
ACGCTCGACAAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT130.10442605831793718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)