FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13676
Sequences flagged as poor quality0
Sequence length53-187
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT613044.823047674758705No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG389328.465925709271716No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG11218.196841181632056No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC5604.094764551038315No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT3002.193623866627669No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATNTAAACTTGTGGTAGTT1731.2649897630886224No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGCT1431.0456273764258555No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATAATAAACTTGTGGTAGT690.5045334893243638No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATNTAAACTTGTGGTAGTTG680.49722140976893825No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAAACTTGTGGTAGT460.33635565954957586No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATAATAAACTTGTGGTAG400.2924831822170225No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT340.24861070488446912No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC320.23398654577361802No Hit
ACGAGTGCGTTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCTG320.23398654577361802No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA300.2193623866627669No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAACTTGTGGTAGTTGG260.19011406844106463No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAATAGATTCTGAATTAG260.19011406844106463No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG230.16817782977478793No Hit
ACGAGTGCGTTTGTTGGATCATATTCGTCCACAAAATAGATTCTGAATTA210.15355367066393683No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG200.14624159110851126No Hit
ACGAGTGCGTTTGTTGGATCATATTTGTCCACAAAATGATTCTGAATTAG170.12430535244223456No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGATATAACTTGTGGTAGTTGGAG150.10968119333138344No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGAATATAACTTGTGGTAGTTGGA140.10236911377595789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)