FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8752
Sequences flagged as poor quality0
Sequence length53-228
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT390244.58409506398537No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT300734.35786106032907No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT4865.553016453382084No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG3704.227605118829982No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT3013.4392138939670933No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAACAAGATTT830.9483546617915904No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAAAACAAGA340.38848263254113347No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGAATATAAACTTGTGGTAGTTGG210.23994515539305303No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT170.19424131627056673No Hit
ACGAGTGCGTGAATGGTCTGCACCAGTAATATGCATATTAAAACAAGATT170.19424131627056673No Hit
ACGAGTGCGTGAATGGTCCTGCACTAGTAATATGCATATTAAAACAAGAT160.18281535648994515No Hit
ACGAGTGCGTGAATGGTCCTGCATCAGTAATATGCATATTAAAACAAGAT150.17138939670932357No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAAACTTGTGGTAG120.13711151736745886No Hit
ACGAGTGCGTGCATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT120.13711151736745886No Hit
ACGAGTGCGTGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGAT110.1256855575868373No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAAGT110.1256855575868373No Hit
ACGAGTGCGTGAATGGTTCTGCACCAGTAATATGCATATTAAAACAAGAT110.1256855575868373No Hit
ACGAGTGCGTAGGCCTGCTGAAATAGACTGAATATAAACTTGTGGTAGTT90.10283363802559414No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAAACAGGAT90.10283363802559414No Hit
ACGAGTGCGTCGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT90.10283363802559414No Hit
ACGAGTGCGTGACTGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT90.10283363802559414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)