FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6825
Sequences flagged as poor quality0
Sequence length55-363
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA302844.366300366300365No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT247736.29304029304029No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT1732.5347985347985347No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGCCTATCCCCTGTGTGCCTTG1341.9633699633699635No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGCCAAGGCACACAGGGGATAG1041.5238095238095237No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT540.7912087912087912No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG410.6007326007326007No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTAGTGGTAG300.43956043956043955No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGACCAGTCATTTTCAGCAGGCCTACGCACTCGT190.2783882783882784No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAAGTAAAGTTCCCATATTAA180.26373626373626374No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGCTTTTCCAAAAAGTGCCTT180.26373626373626374No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGCCAAGGCACACAGGGGATAGG160.23443223443223443No Hit
ACGAGTGCGTACCCTGACATATTCCCAAGGAAAGTAAAGTTCCCATATTA140.20512820512820512No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATAGACTGAATATAAACTTAGTGGTA130.19047619047619047No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGCCAGTCATTTTCAGCAGGCCTACGCACTCGT130.19047619047619047No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATAGACTGAATAATAAACTTAGTGGT120.1758241758241758No Hit
ACGAGTGCGTATCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA100.14652014652014653No Hit
ACGAGTGCGTACCTTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA100.14652014652014653No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGTCCCTTGGGAGTATGTCAGGGTACGCACTCGT100.14652014652014653No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAACTAGTAAACTTGTGGTAG100.14652014652014653No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGCCTATCCCTGTGTGCCTTGG100.14652014652014653No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATAATAAACTTAGTGGTA90.13186813186813187No Hit
ACGAGTGCGTACCCTGACATACTTCCAAGGAAAGTAAAGTTCCCATATTA90.13186813186813187No Hit
ACGAGTGCGTACTCTGACATACTCCCAAGGAAAGTAAAGTTCCCATATTA90.13186813186813187No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGAACGTAAAGTTCCCATTAT80.11721611721611722No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTAGTGGTAGT80.11721611721611722No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGCACTTTTTGGAAAGCCAGTCATTTTCAGCAGGCCTACGCACTCGT80.11721611721611722No Hit
ACGAGTGCGTACCCTGACATACTCTCAAGGAAAGTAAAGTTCCCATATTA80.11721611721611722No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGCACTTTTTGGAAAAGCCAGT80.11721611721611722No Hit
ACGAGTGCGTACCCTGACATACTCCAAGGAAAGTAAAGTTCCCATATTAA80.11721611721611722No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGGACCAGTTTACGGTGCCAGT80.11721611721611722No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGCGTACCCTTGGGAGTATGTCAGGGTACGCACTCGT80.11721611721611722No Hit
ACGAGTGCGTACCCTGACATACTCCCAAGGAAAGTAAAGTTCCCATTATT80.11721611721611722No Hit
ACGAGTGCGTACCCTGATATACTCCCAAGGAAAGTAAAGTTCCCATATTA70.10256410256410256No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGCCAAGGCACACAGGGGGATA70.10256410256410256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)