FastQCFastQC Report
Tue 7 May 2024
EGAF00000127561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6390
Total Bases886.4 kbp
Sequences flagged as poor quality0
Sequence length48-159
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT370657.99687010954616No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG110417.27699530516432No Hit
ACGCTCGACAAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG3986.228482003129891No Hit
ACGCTCGACAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT2013.145539906103286No Hit
ACGCTCGACAAGGCCTGCTGAAATGACTGAATATAACTTGTGGTAGTTGG1892.9577464788732395No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAAATGATTCTGAATTAGC1221.9092331768388107No Hit
ACGCTCGACAAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT951.486697965571205No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAATGATTCTGAATTAGCT390.6103286384976526No Hit
ACGCTCGACAAGGCCTGCTGAATGACTGAATATAACTTGTGGTAGTTGGA310.48513302034428796No Hit
ACGCTCGACATTGTTGGATCATAATTCGTCCACAAAATGATTCTGAATTA150.2347417840375587No Hit
ACGCTCGACAAGGCCTGCTGAAATAGACTGAATATAACTTGTGGTAGTTG140.2190923317683881No Hit
ACGCTCGACAAGGCCTGCTGAAAAATAGACTGAATATAAACTTGTGGTAG130.20344287949921755No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATAATAAACTTGTGGTAGT120.18779342723004694No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAAAAATGATTCTGAATTA120.18779342723004694No Hit
ACGCTCGACAAGGCCTGCTGAACATGACTGAATATAAACTTGTGGTAGTT120.18779342723004694No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAACTCGTGGTAGTT100.1564945226917058No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTC100.1564945226917058No Hit
ACGCTCGACAAGGCCTGCTGAAAATGCCTGAATATAAACTTGTGGTAGTT100.1564945226917058No Hit
ACGCTCGACAAGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG80.12519561815336464No Hit
ACGCTCGACAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG80.12519561815336464No Hit
ACGCTCGACAAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT80.12519561815336464No Hit
ACGCTCGACAAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT80.12519561815336464No Hit
ACGCTCGACAAGGCCTGCTGACAATGACTGAATATAAACTTGTGGTAGTT70.10954616588419405No Hit
ACGCTCGACAAGGCCTGCTGAAAATAGACTGAATATAAACTTAGTGGTAG70.10954616588419405No Hit
ACGCTCGACATTGTTGGATCATATTCGTCCACAATAGATTCTGAATTAGC70.10954616588419405No Hit
ACGCTCGACACGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT70.10954616588419405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)