FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15900
Sequences flagged as poor quality0
Sequence length49-207
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT940159.12578616352201No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT300718.91194968553459No Hit
ACGCTCGACAAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG168110.572327044025156No Hit
ACGCTCGACAAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT2891.8176100628930818No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGGTGCAGGACCATTCTGTCGAGCGT2301.4465408805031448No Hit
ACGCTCGACAAGGCCTGCTGAAATGACTGAATATAACTTGTGGTAGTTGG890.559748427672956No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT870.5471698113207547No Hit
ACGCTCGACAAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT680.42767295597484273No Hit
ACGCTCGACAAGGCCTGCTGAAATGACTGGTGCAGGACCATTCTGTCGAGCGT600.37735849056603776No Hit
ACGCTCGACAAGGCCTGCTGAATGACTGAATATAACTTGTGGTAGTTGGA420.2641509433962264No Hit
ACGCTCGACAGAATGGTCCTGCAACCAGTAATATGCATATTAAAACAAGA410.2578616352201258No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTCATTTTCAGCAGGCCTTGTCGAGCGT310.19496855345911948No Hit
ACGCTCGACAAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT270.16981132075471697No Hit
ACGCTCGACAAGGCCTGTTGAAAATGACTGAATATAAACTTGTGGTAGTT240.1509433962264151No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAAAAACAAGA230.14465408805031446No Hit
ACGCTCGACAAGGCCTGCTGACAATGACTGAATATAAACTTGTGGTAGTT190.11949685534591195No Hit
ACGCTCGACAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAAGT190.11949685534591195No Hit
ACGCTCGACAAGGCCTGCTGAACATGACTGAATATAAACTTGTGGTAGTT170.10691823899371068No Hit
ACGCTCGACAAGGCCTGCTGAAAAATAGACTGAATATAAACTTGTGGTAG160.10062893081761005No Hit
ACGCTCGACAGAATGGTCCTGCACCAGTAATATGCATATTAAAACTAGAT160.10062893081761005No Hit
ACGCTCGACAAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT160.10062893081761005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)