FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000127565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000127565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20638
Sequences flagged as poor quality0
Sequence length46-344
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT1045850.67351487547243No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGAT430820.87411570888652No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAAACTTGTGGTAGTTG18008.721775365830023No Hit
ACGAGTGCGTAGGCCTGCTGAAAATGACTGGTGCAGGACCATTCACGCACTCGT8694.210679329392383No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGGTGCAGGACCATTCACGCACTCGT4202.0350809186936716No Hit
ACGAGTGCGTAGGCCTGCTGAAAAATGACTGAATATAAACTTGTGGTAGT2901.4051749200503925No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTCATTTTCAGCAGGCCTACGCACTCGT2151.0417676131408082No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATATGCATATTAAACAAGATT1180.571760829537746No Hit
ACGAGTGCGTAGGCCTGCTGAAAATAGACTGAATATAAACTTGTGGTAGT850.41186161449752884No Hit
ACGAGTGCGTAGGCCTGCTGAAAATTACTGGTGCAGGACCATTCACGCACTCGT850.41186161449752884No Hit
ACGAGTGCGTAGGCCTGCTGAAATGACTGAATATAACTTGTGGTAGTTGG850.41186161449752884No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGAATATAACTTGTGGTAGTTGGA730.35371644539199537No Hit
ACGAGTGCGTGAATGGTCCTGCAACCAGTAATATGCATATTAAAACAAGA520.25196239945731175No Hit
ACGAGTGCGTAGGCCTGCTGAATGACTGGTGCAGGACCATTCACGCACTCGT470.22773524566333947No Hit
ACGAGTGCGTAGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG350.169590076557806No Hit
ACGAGTGCGTAGGCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTG350.169590076557806No Hit
ACGAGTGCGTAGGCCTGCTGAAATTACTGGTGCAGGACCATTCACGCACTCGT320.15505378428142264No Hit
ACGAGTGCGTAGGCTTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT300.14536292276383372No Hit
ACGAGTGCGTAGGTCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTT280.1356720612462448No Hit
ACGAGTGCGTAGGCCTGCTGAAACTGACTGAATATAAACTTGTGGTAGTT250.12113576896986142No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTCATTTTCAGCAGGACCTACGCACTCGT240.11629033821106696No Hit
ACGAGTGCGTGAATGGTCTTGCACCAGTAATATGCATATTAAAACAAGAT240.11629033821106696No Hit
ACGAGTGCGTGAATGGTCCTGCACCAGTAATTTTCAGCAGGCCTACGCACTCGT220.10659947669347805No Hit
ACGAGTGCGTAGGCCTGTTGAAAATGACTGAATATAAACTTGTGGTAGTT210.1017540459346836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)